Detection of Clarithromycin-resistant Strains from Clinical Isolates of Mycobacterium abscessus.
10.4046/trd.2008.64.6.422
- Author:
Seung Heon LEE
1
;
Young Kil PARK
;
Sung Weon RYO
;
Myung Sup SHIM
;
Woo Jin LEW
;
Hee Jin KIM
Author Information
1. Korean Institute of Tuberculosis, Seoul, Korea. hatchingbird@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium abscessus;
Reverse hybridization assay;
Broth microdilution test;
23S rRNA;
Clarithromycin
- MeSH:
Anti-Bacterial Agents;
Azithromycin;
Chimera;
Clarithromycin;
Mycobacterium;
Point Mutation
- From:Tuberculosis and Respiratory Diseases
2008;64(6):422-426
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. METHODS: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). RESULTS: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. CONCLUSION: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.
