Prevalence of Porphyromonas gingivalis fimA genotypes in the peri-implant sulcus of Koreans assessed using a new primer.
10.5051/jpis.2016.46.1.35
- Author:
Sung Geun KIM
1
;
Ji Youn HONG
;
Seung Il SHIN
;
Ji Hoi MOON
;
Jin Yong LEE
;
Yeek HERR
Author Information
1. Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea. yherr@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Fimbriae;
Peri-implantitis;
Periodontal diseases;
Virulence factors
- MeSH:
DNA;
Genotype*;
Hemorrhage;
Peri-Implantitis;
Periodontal Diseases;
Polymerase Chain Reaction;
Porphyromonas gingivalis*;
Porphyromonas*;
Prevalence*;
Risk Factors;
Virulence;
Virulence Factors
- From:Journal of Periodontal & Implant Science
2016;46(1):35-45
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Porphyromonas gingivalis fimA is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. We aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of P. gingivalis fimA genotypes in Koreans using a new primer. METHODS: A total of 248 plaque samples were taken from the peri-implant sulci of 184 subjects. The control group consisted of sound implants with a peri-implant probing depth (PD) of 5 mm or less with no bleeding on probing (BOP). Test group I consisted of implants with a peri-implant PD of 5 mm or less and BOP, and test group II consisted of implants with a peri-implant PD of more than 5 mm and BOP. DNA was extracted from each sample and analyzed a using a polymerase chain reaction (PCR) with P. gingivalis-specific primers, followed by an additional PCR assay to differentiate the fimA genotypes in P. gingivalis- positive subjects. RESULTS: The Prevalence of P. gingivalis in each group did not significantly differ (P>0.05). The most predominant fimA genotype in all groups was type II. The prevalence of type Ib fimA was significantly greater in test group II than in the control group (P<0.05). CONCLUSIONS: The fimA type Ib genotype of P. gingivalis was found to play a critical role in the destruction of peri-implant tissue, suggesting that it may be a distinct risk factor for peri-implantitis.
