Value of Elisa for finding the specific antibody IgG with the anti-M tuberculosis property in diagnosis of the infiltrative pulmonary tuberculosis in adults
- Author:
Huan Van Hoang
- Publication Type:Journal Article
- Keywords:
pulmonary tuberculosis
- MeSH:
Tuberculosis, Pulmonary;
Enzyme-Linked Immunosorbent Assay
- From:Journal of Practical Medicine
2002;435(11):55-57
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Purpose: assessing the effectiveness of ELISA using sonicated antigen of M. tuberculosis in the diagnosis of adult infiltrative pulmonary tuberculosis. Materials: 102 patients from 18 to 70 years old were diagnosed as preliminary infiltrative tuberculosis by clinic and X-ray. And then, 58 patients of them with infiltrative tuberculosis according to the criteria of WHO (1997) and Chebanov V-1980, the rest(control) with lung disease non tuberculosis (4 pneumonie, 6 abcess, 34 lung tumors). Methods: serum of 102 patients were ELISA tested for IgG antibody against sonicated antigen of M. tuberculosis OD= 0,5 was a cut off value for IgG measurements (Charpin D. 1995). Chest-X-ray-images were analyzed by Pr. Bui Xuan Tam and Dr. Hoang Van Huan. Results: 1. The mean IgG value in the infiltrative tuberculosis group was significantly higher than non-tuberculosis group (p<0.001) (0.863+/-0.395 vs 0.252+/-0.19). 2. The larger new tuberculosis lessions on chest-X-ray, the stronger humoral immuno response (P< 0.01), that did not related to Mantoux test. 3. Test ELISA using sonicated antigen of M. tuberculosis, having the sensitivity 81.03%, the specificity 95.45%, the posititive predictive value 95.91%, the negative predictive value 79.24%, the accuracy 87.25%. Conclusion: ELISA can be an useful test for the rapid diagnosis of infiltrative pulmonary tuberculosis.
