TACI:Fc scavenging B cell activating factor (BAFF) alleviates ovalbumin-induced bronchial asthma in mice.
- Author:
Eun Yi MOON
1
;
Sook Kyung RYU
Author Information
1. Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea. eunyimoon@sejong.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
asthma;
B-cell activating factor;
ovalbumin;
transmembrane activator and CAML interactor protein
- MeSH:
Animals;
Apoptosis;
Asthma/chemically induced/*drug therapy/immunology;
B-Cell Activating Factor/*biosynthesis;
Bronchi/metabolism/pathology;
Bronchoalveolar Lavage Fluid/cytology;
Eosinophils/pathology;
Female;
Humans;
Immunoglobulin Fc Fragments/*genetics;
Immunoglobulin G/*genetics;
Lymphocytes/pathology;
Mice;
Mice, Inbred BALB C;
*Ovalbumin;
Pulmonary Alveoli/metabolism;
Recombinant Fusion Proteins/genetics/*therapeutic use;
Spleen/metabolism;
Transmembrane Activator and CAML Interactor Protein/*genetics
- From:Experimental & Molecular Medicine
2007;39(3):343-352
- CountryRepublic of Korea
- Language:English
-
Abstract:
Asthma was induced by the sensitization and challenge with ovalbumin (OVA) in mice. B-cell activating factor (BAFF) plays a role in mature B cell generation and maintenance. Here, we investigated whether, BAFF expression was changed in OVA-induced mice and whether the control of BAFF expression level alleviates the symptom of bronchial asthma. BAFF expression was detected in alveolar-associated cells surrounding bronchi of OVA-induced mouse lung tissues. BAFF protein was also increased in OVA-induced mouse serum. The increased BAFF transcripts was detected in OVA-induced mouse splenocytes. OVA-induced asthma was associated with the increased number of eosinophils in bronchoalveolar lavage fluid (BALF). When TACI:Fc scavenging soluble BAFF was injected to OVA-induced mice, a significant inhibition was detected in the thickness of airway smooth muscle and glycol-containing cellular elements in airway that were visualized by hematoxylin/eosin Y and periodic acid-Schiff staining, respectively. In addition, when mice were treated with TACI:Fc protein, BAFF protein level was decreased in alveolar-associated cells surrounding bronchi of OVA-induced mouse lung tissues compared to control mice. When compared to OVA-induced control, TACI:Fc treatment reduced the percentage of non-lymphoid cells and no changes were detected in lymphoid cell population. Hypodiploid cell formation in BALF was decreased by OVA-challenge but it was recovered by TACI:Fc treatment. Collectively, data suggest that asthmatic symptom could be alleviated by scavenging BAFF and then BAFF could be a novel target for the develpoment of anti-asthmatic agents.
