The Immunomodulatory Activity of Mori folium, the Leaf of Morus alba L., in RAW 264.7 Macrophages In Vitro.
10.15430/JCP.2016.21.3.144
- Author:
Da Hye KWON
1
;
Ji Min CHEON
;
Eun Ok CHOI
;
Jin Woo JEONG
;
Ki Won LEE
;
Ki Young KIM
;
Sung Goo KIM
;
Suhkmann KIM
;
Su Hyun HONG
;
Cheol PARK
;
Hye Jin HWANG
;
Yung Hyun CHOI
Author Information
1. Anti-Aging Research Center, Dongeui University, Busan, Korea. choiyh@deu.ac.kr
- Publication Type:Original Article
- Keywords:
Mori folium;
Macrophages;
Immune response;
Phagocytosis
- MeSH:
Blotting, Western;
Cell Survival;
Cytokines;
Dinoprostone;
Enzyme-Linked Immunosorbent Assay;
In Vitro Techniques*;
Interleukin-10;
Interleukin-6;
Interleukins;
Macrophages*;
Medicine, Traditional;
Methods;
Morus*;
Nitric Oxide;
Nitric Oxide Synthase;
Phagocytosis;
RNA, Messenger;
Water
- From:Journal of Cancer Prevention
2016;21(3):144-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Immunoregulatory elements have emerged as useful immunotherapeutic agents against cancer. In traditional medicine, Mori folium, the leaf of Morus alba L. (Moraceae), has been used for various medicinal purposes; however, the immunomodulatory effects have not been fully identified. We evaluated the immunoenhancing potential of water extract of Mori folium (WEMF) in murine RAW264.7 macrophages. METHODS: RAW264.7 cells were treated with WEMF for 24 hours and cell viability was detected by an MTT method. Nitric oxide (NO) levels in the culture supernatants were assayed using Griess reagent. The productions of prostaglandin E2 (PGE2) and immune-related cytokines was measured using ELISA detection kits. The mRNA and protein expression levels of Inducible NO synthase, COX-2, and cytokines were assayed by reverse transcription-PCR and Western blotting, respectively. The effect of WEMF on phagocytic activity was measured using a Phagocytosis Assay Kit. RESULTS: WEMF significantly stimulated the production of NO and PGE2 as immune response parameters at noncytotoxic concentrations, which was associated with the increased expression of inducible NO synthase and COX-2. The release and expression of cytokines, such as TNF-α, interleukin (IL)-1β, IL-6, and IL-10, were also significantly increased in response to treatment with WEMF. Moreover, WEMF promoted the macrophagic differentiation of RAW264.7 cells and the resulting phagocytosis activity. CONCLUSIONS: WEMF has the potential to modulate the immune function by regulating immunological parameters. Further studies are needed to identify the active compounds and to support the use of WEMF as an immune stimulant.
