Effects of S-Adenosylmethionine and Its Combinations With Taurine and/or Betaine on Glutathione Homeostasis in Ethanol-induced Acute Hepatotoxicity.
10.15430/JCP.2016.21.3.164
- Author:
Seo Yeon LEE
1
;
Kwang Suk KO
Author Information
1. Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea. kko@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
S-adenosylmethionine;
Taurine;
Betaine;
Ethanol;
Glutathione
- MeSH:
Alanine Transaminase;
Animals;
Aspartate Aminotransferases;
Betaine*;
Catalytic Domain;
Cell Survival;
Ethanol;
Glutamate-Cysteine Ligase;
Glutathione Synthase;
Glutathione*;
Homeostasis*;
Liver;
Mice;
Oxidative Stress;
Oxidoreductases;
Risk Factors;
RNA, Messenger;
S-Adenosylmethionine*;
Taurine*
- From:Journal of Cancer Prevention
2016;21(3):164-172
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Exposure to ethanol abuse and severe oxidative stress are risk factors for hepatocarcinoma. The aim of this study was to evaluate the effects of S-adenosylmethionine (SAMe) and its combinations with taurine and/or betaine on the level of glutathione (GSH), a powerful antioxidant in the liver, in acute hepatotoxicity induced by ethanol. METHODS: To examine the effects of SAMe and its combinations with taurine and/or betaine on ethanol-induced hepatotoxicity, AML12 cells and C57BL/6 mice were pretreated with SAMe, taurine, and/or betaine, followed by ethanol challenge. Cell viability was detected with an MTT assay. GSH concentration and mRNA levels of GSH synthetic enzymes were measured using GSH reductase and quantitative real-time reverse transcriptase-PCR. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured with commercially available kits. RESULTS: Pretreatment of SAMe, with or without taurine and/or betaine, attenuated decreases in GSH levels and mRNA expression of the catalytic subunit of glutamate-cysteine ligase (GCL), the rate-limiting enzyme for GSH synthesis, in ethanol-treated cells and mice. mRNA levels of the modifier subunit of GCL and glutathione synthetase were increased in mice treated with SAMe combinations. SAMe, taurine, and/or betaine pretreatment restored serum ALT and AST levels to control levels in the ethanol-treated group. CONCLUSIONS: Combinations of SAMe with taurine and/or betaine have a hepatoprotective effect against ethanol-induced liver injury by maintaining GSH homeostasis.
