Gingival absorption of α-tocopherol acetate and 18β-glycyrrhetinic acid : in vitro evaluation in reconstructed gingival tissue.
10.11149/jkaoh.2016.40.2.79
- Author:
Yun Sun KIM
1
;
Ju Ae KIM
;
Aram YOU
;
Hosong CHO
;
Jae Young SHIN
;
Sanghwa LEE
Author Information
1. Research Park, LG Household & Healthcare Ltd., Daejeon, Korea. shleek@lgcare.com
- Publication Type:Original Article
- Keywords:
Absorption;
α-Tocopherol acetate;
18β-Glycyrrhetinic acid;
Periodontal disease;
Toothpaste
- MeSH:
Absorption*;
Chromatography, Liquid;
In Vitro Techniques*;
Periodontal Diseases;
Tissue Extracts;
Toothpastes
- From:Journal of Korean Academy of Oral Health
2016;40(2):79-84
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: To assess the absorption of α-tocopherol acetate and 18β-glycyrrhetinic acid, which are used as active ingredients in toothpaste, into a reconstructed gingival tissue. METHODS: EpiGingival™ tissues were treated with a 25% slurry of toothpaste containing 2% α-tocopherol acetate and 0.3% 18β-glycyrrhetinic acid, for 2 minutes. The treatment was repeated up to 6 times, with 1 hour intervals. After completion of all treatments, the active ingredients in the tissue extracts and receiver solutions were measured by high performance liquid chromatography. RESULTS: Although α-tocopherol acetate was not detected, α-tocopherol was detected in the tissue extracts, indicating that α-tocopherol acetate was bioconverted to α-tocopherol after absorption. We could detect 18β-glycyrrhetinic acid both in the tissue extracts and in the receiver solutions, with a positive correlation to the number of treatments. CONCLUSIONS: We found that our toothpaste effectively delivered α-tocopherol acetate and 18β-glycyrrhetinic acid to a reconstructed gingival tissue in vitro.
