Reduction of Influenza A (H1N1) Virus Through a Leukoreduction Filter.
- Author:
Ga Eun CHO
1
;
Jung Hwan KIM
;
In Bum SUH
Author Information
1. Institute of Medical Science, School of Medicine, Kangwon National University, Chuncheon, Korea.
- Publication Type:Original Article
- Keywords:
New influenza A (H1N1) virus;
RNA;
Filter;
Leukoreduction;
Transfusion
- MeSH:
Blood Group Incompatibility;
Communicable Diseases;
Cytomegalovirus Infections;
Filtration;
Genome;
Influenza, Human;
Plasma;
RNA;
Viruses
- From:Korean Journal of Blood Transfusion
2010;21(1):65-73
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Leukoreduction can reduce the risk of HLA alloimmunization, recurrent febrile nonhemolytic transfusion reactions, and several transfusion-transmitted infectious diseases, including cytomegalovirus infection. Transmission of the new influenza A (H1N1) virus through transfusion may be a concern. We evaluated the effect of filtration with a leukoreduction filter on H1N1 genomes. METHODS: To evaluate the effect of filtration by a leukoreduction filter on H1N1 genomes, we analyzed pre- and post-filtered samples from nasopharyngeal swabs and 10 positive plasma samples using real time RT-PCR. RESULTS: The 10 samples (nasopharyngeal swabs and plasma) contained H1N1 RNA, and filtration with a leukoreduction filter reduced these levels (threshold cycle values from 31.42+/-2.06 to 38.84+/-1.47 in nasopharyngeal swabs, from 35.63+/-2.19 to 39.38+/-2.65 in plasma samples). CONCLUSION: Filtration with a leukoreduction filter can reduce H1N1 genome levels, but may not be completely sufficient for total eradication of this pathogen.
