Auto-antibody Showing Anti-Fy(b) Specificity as Proven by the Dilution Method in the Presence of Warm Autoantibodies: A Case Report.
- Author:
Eun Sun JEONG
1
;
Min Joong JANG
;
Duck CHO
;
Chae Seung LIM
;
Dong Wook RYANG
Author Information
1. Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. dcho@chonnam.ac.kr
- Publication Type:Case Report
- Keywords:
Anti-Fy(b);
Dilution method;
Warm autoantibody
- MeSH:
Autoantibodies;
Dithiothreitol;
Erythrocytes;
Genotype;
Humans;
Indicator Dilution Techniques;
Lupus Erythematosus, Systemic;
Papain;
Phenotype;
Polyethylene Glycols;
Sensitivity and Specificity*
- From:Korean Journal of Blood Transfusion
2010;21(1):74-79
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Several approaches have been introduced to detect allo-antibodies in the presence of warm auto-antibodies, and these methods include warm autoadsorption, cysteine-activated papain and dithiothreitol (ZZAP), and polyethylene glycol (PEG) and dilution of the patient's serum. Among them, the dilution technique is a simple and rapid method. During pretransfusion testing of a 33 year-old systemic lupus erythematosus (SLE) patient with warm auto-antibodies, antibody identification was done by the dilution technique with using serum diluted 1-in-8. The patient demonstrated an anti-Fy(b) pattern of reactivity in his sera. Contrary to our expectations, the phenotype of the erythrocytes was Fy(a+/b+) and the genotype, as assessed by performing PCR-restriction fragment length polymorphism (RFLP), was FY*A/FY*B. These results suggest that the antibody is an autoantibody showing anti-Fy(b) specificities. An antibody identification test using undiluted serum showed the same result when 40 days had passed. We report here on a case with auto-anti-Fy(b) proven by the dilution method in the presence of warm autoantibodies.
