The expression and cellular localization of phospholipase D isozymes in the developing mouse testis.
- Author:
Seungjoon KIM
1
;
Heechul KIM
;
Yongduk LEE
;
Jin Won HYUN
;
Young Ho LEE
;
Min Kyoung SHIN
;
Do Sik MIN
;
Taekyun SHIN
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: cellular localization; mouse; phospholipase D; spermatogenesis; testis
- MeSH: Animals; Blotting, Western; Female; Immunohistochemistry; Isoenzymes; Male; Mice; Mice, Inbred BALB C; Phospholipase D/biosynthesis/*metabolism; Spermatogenesis/physiology; Testis/*enzymology/growth & development
- From:Journal of Veterinary Science 2007;8(3):209-212
- CountryRepublic of Korea
- Language:English
- Abstract: To examine the involvement of phospholipase D (PLD)isozymes in postnatal testis development, the expression ofPLD1 and PLD2 was examined in the mouse testis atpostnatal weeks 1, 2, 4, and 8 using Western blot analysisand immunohistochemistry. The expression of both PLD1and PLD2 increased gradually with development frompostnatal week 1 to 8. Immunohistochemically, PLDimmunoreactivity was detected in some germ cells in thetestis and interstitial Leydig cells at postnatal week 1.PLD was mainly detected in the spermatocytes andresidual bodies of spermatids in the testis after 8 weeksafter birth. The intense immunostaining of PLD in Leydigcells remained unchanged by postnatal week 8. Thesefindings suggest that PLD isozymes are involved in thespermatogenesis of the mouse testis.
