Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant.
- Author:
Kiju KIM
1
;
Soyeon PARK
;
Youngjae CHO
;
Jeong Yeon KWAK
;
Zheng Wu KANG
;
Eun Hee KIM
;
Hwan Won CHOI
;
Ho Keun WON
;
Yun Hee NOH
;
Tae Wook HAHN
Author Information
1. College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. twhahn@kangwon.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
cross protection;
polyphosphate kinase deleted mutant;
Salmonella Enteritidis;
temperature-sensitive mutant
- MeSH:
Animals;
Antibody Formation;
Cecum;
Chickens;
Colon;
Cross Protection;
Liver;
Mice;
Phosphotransferases;
Salmonella enteritidis*;
Salmonella*;
Spleen
- From:Korean Journal of Veterinary Research
2013;53(4):211-216
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.
