Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice.
- Author:
Dong Joo AHN
1
;
Jung Won YOUM
;
Suk Weon KIM
;
Won Kee YOON
;
Hyoung Chin KIM
;
Tai Young HUR
;
Young Hee JOUNG
;
Jae Heung JEON
;
Hyun Soon KIM
Author Information
1. Plant System Engineering Center, KRIBB, 125 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea. hyuns@kribb.re.kr
- Publication Type:Original Article
- Keywords:
oral administration;
potato-derived antigen;
transmissible gastroenteritis virus;
transgenic plant
- MeSH:
Administration, Oral;
Animals;
Blotting, Northern;
Blotting, Western;
Coronavirus;
DNA;
Enzyme-Linked Immunosorbent Assay;
Escherichia;
Gastroenteritis;
Glycoproteins*;
Immunoglobulin A;
Immunoglobulins;
Mice*;
Plants, Genetically Modified;
Polymerase Chain Reaction;
RNA, Messenger;
Solanum tuberosum*;
Transmissible gastroenteritis virus*;
Vaccines
- From:Korean Journal of Veterinary Research
2013;53(4):217-224
- CountryRepublic of Korea
- Language:English
-
Abstract:
Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-S0.7) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-S0.7 gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-S0.7 protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-S0.7 antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.
