Evaluation of cytolytic activity and phenotypic changes of circulating blood immune cells in patients with colorectal cancer by a simple preparation of peripheral blood mononuclear cells.
10.4174/jkss.2013.85.5.230
- Author:
Jae Cheol KIM
1
;
Joungbum CHOI
;
Su Jin LEE
;
Yun A LEE
;
Young Min JEON
;
Yong Won KANG
;
Jong Kyun LEE
Author Information
1. Immunology Laboratory, Seniors Inje Hospital, Holon Center, Inje, Korea. gskjc@hanmail.net
- Publication Type:Original Article
- Keywords:
Blood cells;
Natural killer cells;
Immunologic cytotoxicity;
Colorectal neoplasms
- MeSH:
Antibodies, Monoclonal;
Blood Cells;
Colorectal Neoplasms*;
Cytotoxicity, Immunologic;
Fluorescence;
Humans;
Immunity, Cellular;
K562 Cells;
Killer Cells, Natural;
L-Lactate Dehydrogenase;
Lymphocytes;
Phenotype;
Tissue Donors
- From:Journal of the Korean Surgical Society
2013;85(5):230-235
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aimed to assess the cytolytic activity and the phenotype of circulating blood immune cells in cancer patients by using a simple preparation of peripheral blood mononuclear cells (PBMCs). METHODS: Peripheral blood was obtained from 94 diagnosed colorectal cancer (CRC) patients and 112 healthy donors. PBMCs were cocultured with K562 cells for 2 hours and lactate dehydrogenase released from the dead K562 cells was measured by using a spectrophotometer. Meanwhile, PBMCs were stained with fluorescence conjugated monoclonal antibodies (mAbs) and analyzed by flow cytometry. RESULTS: The cytolytic activity of PBMCs were significantly different between CRC patient and healthy groups (8.82% +/- 3.84% vs. 17.51% +/- 8.57%; P < 0.001). However, no significant difference in the cytolytic activity was observed after surgery in the CRC patient group (before surgery, 8.82% +/- 3.84% vs. after surgery, 9.95% +/- 4.94%; P = 0.326). In addition, the percentage of peripheral blood natural killer cells was significantly higher in the preoperative patient group than in the healthy group (19.97% +/- 11.51% vs. 15.60% +/- 5.77%, P = 0.041). In contrast, the percentage of peripheral blood lymphocytes was lower in the preoperative patient group than in the healthy group (28.41% +/- 8.31% vs. 36.4% +/- 8.6%, P < 0.001). CONCLUSION: These results demonstrate that circulating blood immune cells of CRC patients are functionally impaired and undergo an immunophenotypic perturbation, and show that a simple preparation of PBMCs can be useful to evaluate cellular immunity in cancer.
