alpha-Mangostin Reduced ER Stress-mediated Tumor Growth through Autophagy Activation.
- Author:
Sung Jin KIM
1
;
Eun Hye HONG
;
Bo Ra LEE
;
Moon Ho PARK
;
Ji Won KIM
;
A Rim PYUN
;
Yeon Jeong KIM
;
Sun Young CHANG
;
Young Won CHIN
;
Hyun Jeong KO
Author Information
- Publication Type:Original Article
- Keywords: Autophagy; alpha-Mangostin; Thapsigargin; ER stress; Antitumor activity; Colon cancer
- MeSH: Animals; Autophagy; Calcium-Transporting ATPases; Cell Proliferation; Colonic Neoplasms; Epithelial Cells; Fruit; Garcinia mangostana; Mice; Mice, Transgenic; Phosphorylation; Reticulum; Thapsigargin; Transplants; Xanthones
- From:Immune Network 2012;12(6):253-260
- CountryRepublic of Korea
- Language:English
- Abstract: alpha-Mangostin is a xanthon derivative contained in the fruit hull of mangosteen (Garcinia mangostana L.), and the administration of alpha-Mangostin inhibited the growth of transplanted colon cancer, Her/CT26 cells which expressed Her-2/neu as tumor antigen. Although alpha-Mangostin was reported to have inhibitory activity against sarco/endoplasmic reticulum Ca2+ ATPase like thapsigargin, it showed different activity for autophagy regulation. In the current study, we found that alpha-Mangostin induced autophagy activation in mouse intestinal epithelial cells, as GFP-LC3 transgenic mice were orally administered with 20 mg/kg of alpha-Mangostin daily for three days. However, the activation of autophagy by alpha-Mangostin did not significantly increase OVA-specific T cell proliferation. As we assessed ER stress by using XBP-1 reporter system and phosphorylation of eIF2alpha, thapsigargin-induced ER stress was significantly reduced by alpha-Mangostin. However, coadministration of thapsigargin with alpha-Mangostin completely blocked the antitumor activity of alpha-Mangostin, suggesting ER stress with autophagy blockade accelerated tumor growth in mouse colon cancer model. Thus the antitumor activity of alpha-Mangostin can be ascribable to the autophagy activation rather than ER stress induction.
