The Role of the Ethylacetate Fraction from Hydnocarpi Semen in Acute Inflammation In Vitro Model.
- Author:
Geum Seon LEE
1
;
Hong SHIM
;
Ki Man LEE
;
Seung Hyun KIM
;
Dongsool YIM
;
Jae Hoon CHEONG
;
Tae Jin KANG
Author Information
- Publication Type:In Vitro ; Brief Communication
- Keywords: Hydnocarpi Semen; Ethylacetate fraction; Inflammation; Cytokines
- MeSH: Animals; Cytokines; Diabetic Foot; Fatty Acids; Fibroblasts; Inflammation; Macrophages; Mice; Semen; Skin; Transforming Growth Factor beta; Tumor Necrosis Factor-alpha; Ulcer; Vascular Endothelial Growth Factor A; Wound Healing
- From:Immune Network 2012;12(6):291-295
- CountryRepublic of Korea
- Language:English
- Abstract: We previously reported that Hydnocarpi Semen (HS) has a wound healing effect on diabetic foot ulcer lesion in mice. In this study, ethylacetate (EtOAc) fraction from HS extract were evaluated for their wound healing activity by using in vitro acute inflammation model. GC and GC/MS analysis shows that the main constituents in EtOAc fraction are chaulmoogric acid, hydnocarpic acid, and gorlic acid. EtOAc fraction activated macrophages to increase the production of TNF-alpha. The fraction also increased the production of TGF-beta and VEGF, which induced fibroblast activation and angiogenesis. These results suggest that the mechanism that the fraction helps to enhance healing of skin wound is possibly associated with the production of TNF-alpha, as well as secretion of VEGF, TGF-beta and HS may have a new bioactive material for the treatment of skin wound.
