Comparison of Two Automated Immunoassays for the Detection of Anti-Hepatitis A Virus Total Immunoglobulin and IgM.
- Author:
Sang Yong SHIN
1
;
Hyun Jin LIM
;
Changmin YI
;
Shinae YU
;
Min Jung KWON
;
Hyosoon PARK
;
Young Jae KIM
;
Chae Lim JUNG
;
Hee Yeon WOO
Author Information
1. Department of Laboratory Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea. drwhy@hanmail.net
- Publication Type:Original Article
- Keywords:
Hepatitis A virus;
Immunoglobulins;
ADVIA Centaur XP;
MODULAR ANALYTICS E170
- MeSH:
Hepatitis A;
Hepatitis A Antibodies;
Hepatitis A virus;
Immunoassay;
Immunoglobulin M;
Immunoglobulins;
Luminescence;
Reverse Transcription;
Viruses
- From:Journal of Laboratory Medicine and Quality Assurance
2011;33(2):103-109
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The detection of total anti-hepatitis A virus (anti-HAV) immunoglobulin (Ig) and IgM is important for diagnosing acute hepatitis A. Our laboratory introduced new commercial automated chemiluminescence immunoassays (CLIAs) for use in addition to pre-existing automated CLIA. We evaluated the rate of agreement in the detection of total anti-HAV Ig and IgM in serum samples between two automated CLIAs. METHODS: We analyzed 181 samples those were submitted for testing at Kangbuk Samsung Medical Center. We analyzed the rate of agreement between the ADVIA Centaur XP (Siemens, Germany) and the MODULAR ANALYTICS E170 (Roche, Switzerland) analyzers. We performed reverse transcription (RT)-PCR when there was a discrepancy between the results from the two analyzers. RESULTS: The agreement rates between the ADVIA Centaur XP and the MODULAR ANALYTICS E170 for total anti-HAV Ig and IgM were 97.2% and 98.9%, respectively. Discrepant results were obtained in seven cases; all were found to be HAV-negative based on RT-PCR analysis. CONCLUSIONS: The total anti-HAV Ig and IgM results obtained using the two automated analyzers were comparable. However, in cases of equivocal results tested by the ADVIA Centaur XP for anti-HAV IgM, retesting and follow-up testing of samples are recommended.
