Carbonic Anhyd rase Activity in Muller Cell.
- Author:
Nam Chun CHO
1
;
Eui Yong KWEON
Author Information
1. Department of Ophthalmology, College of Medicine, Chonbuk National University, Korea.
- Publication Type:Original Article
- Keywords:
Carbonic anhydrase;
Muller cell;
Metabolism
- MeSH:
Carbon Dioxide;
Carbon*;
Carbonic Anhydrases;
Ependymoglial Cells;
Homeostasis;
Humans;
Metabolism;
Nerve Fibers;
Retina;
Tissue Donors
- From:Journal of the Korean Ophthalmological Society
2000;41(5):1068-1072
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Carbonic anhydrase, an enzyme catalysing the reversible hydration of carbon dioxide, is present in the Muller cells.Because the enzyme is not present in other uroretinal cells in the retina, it can be used as a marker for Muller cells.Carbonic anhydrase activity was demonstrated bnzymehistochemically in human and rabbit Muller cells to know a relation of metabolic functions and carbonic anhydrase activity.Human retinas were obtained from donor eyes.The eyes were enucleated immediately after death forenzymatic activity. In human retina, heavy staining was found in the inner nuclear layer and nerve fiber layer, moderate staining in the outer nuclear layer and weak or no staining in the plexiform layers.In rabbit retina, heavy staining was found in the nerve fiber layer and nuclear layers and weak reaction in the two plexiform layers. These findings suggest that Muller cells may participate in CO2 homeostasis mechanism of carbonic anhydrase in the retina.
