Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells.
10.3341/jkos.2011.52.12.1514
- Author:
Seung Hee LEE
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Erythropoietin;
Nitric oxide;
Trabecular meshwork cells
- MeSH:
Erythropoietin;
Humans;
Nitric Oxide;
RNA, Messenger;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2011;52(12):1514-1518
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR. RESULTS: EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity. CONCLUSIONS: EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity.
