Effects of Angiotensin II and Shear Stress Interaction on Vascular Inflammation.
10.5646/jksh.2011.17.1.17
- Author:
Sung Hyun CHOI
1
;
Eun Hye PARK
;
Yujin OH
;
Sang Hong BAEK
Author Information
1. Laboratory of Cardiovascular Research, The Catholic University of Korea School of Medicine, Seoul, Korea. whitesh@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Vascular cell adhesion molecule 1;
Intercellular adhesion molecule 1;
Angiotensin II;
Shear stress;
Human aortic endothelial cell
- MeSH:
Angiotensin II;
Angiotensins;
Atherosclerosis;
Benzimidazoles;
Benzoates;
Endothelial Cells;
Humans;
Immunoblotting;
Inflammation;
Intercellular Adhesion Molecule-1;
Vascular Cell Adhesion Molecule-1
- From:Journal of the Korean Society of Hypertension
2011;17(1):17-27
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Angiotensin II (AngII) and abnormal oscillatory shear stress are highly associated with vascular inflammation including atherosclerosis. However, it is poorly understood how interactions between AngII and shear stress in human aortic endothelial cells (HAEC) are involved in mechanisms by which cellular adhesion molecules are expressed. The purpose of this study was to improve that understanding. METHODS: AngII (10(-7)M for 6 hr) and two-types of shear stress treatments were used: laminar shear stress (LS: unidirectional, 12 dynes/cm2) and oscillatory shear stress (OS: bi-directional, 5 dynes/cm2, 1 Hz) in HAEC. Immunoblotting was used to detect expression of cellular adhesion molecules markers such as vascular cell adhesion molecule 1 (VCAM1) and intercellular adhesion molecule 1 (ICAM1). RESULTS: AngII significantly increased VCAM1 and ICAM1 expression in HAEC that had been reduced due to pretreatment with telmisartan. AngII-LS co-stimulation and AngII-OS co-stimulation significantly increased VCAM1 and ICAM1 expression in HAEC. The expression levels of VCAM1 and ICAM1 were also, significantly reduced when pretreated with telmisartan. However, VCAM1 and ICAM1 expression were significantly reduced under LS and OS stimulation. CONCLUSIONS: Telmisartan may modulate the expressions of VCAM1 and ICAM1 via different types of shear stress in HAEC that are activated by AngII.
