The requirement of natural killer T-cells in tolerogenic APCs-mediated suppression of collagen-induced arthritis.
10.3858/emm.2010.42.8.055
- Author:
Sundo JUNG
1
;
Yoon Kyung PARK
;
Jung Hoon SHIN
;
Hyunji LEE
;
Soo Young KIM
;
Gap Ryol LEE
;
Se Ho PARK
Author Information
1. School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. sehopark@korea.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
antigen-presenting cells;
antigens;
arthritis;
CD1d;
experimental;
immune tolerance;
natural killer T-cells
- MeSH:
Animals;
Antibodies/blood;
Antibody Formation/immunology;
Antibody Specificity/immunology;
Antigen-Presenting Cells/*immunology;
Antigens, CD1d/immunology;
Arthritis, Experimental/blood/*immunology/*prevention & control;
Collagen Type II/immunology;
Cytokines/blood;
Immune Tolerance/*immunology;
Inflammation Mediators/blood;
Mice;
Natural Killer T-Cells/*immunology;
Th1 Cells/immunology
- From:Experimental & Molecular Medicine
2010;42(8):547-554
- CountryRepublic of Korea
- Language:English
-
Abstract:
TGF-beta-induced tolerogenic-antigen presenting cells (Tol-APCs) could induce suppression of autoimmune diseases such as collagen-induced arthritis (CIA) and allergic asthma. In contrast, many studies have shown that NKT cells are involved in the pathogenesis of Th1-mediated autoimmune joint inflammation and Th2-mediated allergic pulmonary inflammation. In this study, we investigated the effect of CD1d-restricted NKT cells in the Tol-APCs-mediated suppression of autoimmune disease using a murine CIA model. When CIA-induced mice were treated with Tol-APCs obtained from CD1d+/- or CD1d-/- mice, unlike CD1d+/- APCs, CD1d-/- Tol-APCs failed to suppress CIA. More specifically, CD1d-/- Tol-APCs failed to suppress the production of inflammatory cytokines and the induction of Th2 responses by antigen-specific CD4 T cells both in vitro and in vivo. Our results demonstrate that the presence of CD1d-restricted NKT cells is critical for the induction of Tol-APCs-mediated suppression of CIA.
