Induction of Potent Antigen-specific Cytotoxic T Cell Response by PLGA-nanoparticles Containing Antigen and TLR Agonist.
- Author:
Young Ran LEE
1
;
Young Hee LEE
;
Ki Hyang KIM
;
Sun A IM
;
Chong Kil LEE
Author Information
- Publication Type:Brief Communication
- Keywords: PLGA; Nanoparticle; Poly-IC; CpG; CTL; Antitumor activity
- MeSH: Animals; Dendritic Cells; Immunization; Immunoglobulin G; Lactic Acid; Mice; Nanoparticles; Ovalbumin; Ovum; Peptides; Polyglycolic Acid; Polymers; Veins
- From:Immune Network 2013;13(1):30-33
- CountryRepublic of Korea
- Language:English
- Abstract: Previously we showed that biodegradable nanoparticles containing poly-IC or CpG oligodeoxynucleotide (ODN) together with ovalbumin (OVA) were efficient at inducing MHC-restricted presentation of OVA peptides in dendritic cells. The CTL-inducing activities of the nanoparticles were examined in the present study. Nanoparticles containing poly-IC or CpG ODN together with OVA were prepared using biodegradable polymer poly(D,L-lactic acid-co-glycolic acid), and then were opsonized with mouse IgG. The nanoparticles were injected into the tail vein of mice, and 7 days later the OVA-specific CTL activities were measured using an in vivo CTL assay. Immunization of mice with the nanoparticles containing poly-IC or CpG ODN together with OVA elicited potent OVA-specific CTL activity compared to those containing OVA only. In accordance with these results, nanoparticles containing poly-IC or CpG ODN together with OVA exerted potent antitumor activity in mice that were subcutaneously implanted with EG7.OVA tumor cells. These results show that encapsulation of poly-IC or CpG ODN together with antigen in biodegradable nanoparticles is an effective approach for the induction of potent antigen-specific CTL responses in vivo.
