Rhamnogalacturonan II is a Toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells.
- Author:
Sung Nam PARK
1
;
Kyung Tae NOH
;
Young Il JEONG
;
In Duk JUNG
;
Hyun Kyu KANG
;
Gil Sun CHA
;
Su Jung LEE
;
Jong Keun SEO
;
Dae Hwan KANG
;
Tae Ho HWANG
;
Eun Kyung LEE
;
Byungsuk KWON
;
Yeong Min PARK
Author Information
1. Department of Microbiology and Immunology, School of Medicine, Pusan National University, Yangsan, South Korea. immunpym@pusan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
adjuvant;
dendritic cells;
rhamnogalacturonan II;
Toll-like receptor 4;
tumor
- MeSH:
Acute-Phase Proteins/metabolism;
Adaptor Proteins, Vesicular Transport/metabolism;
Animals;
Antigens, CD14/metabolism;
Bone Marrow Cells/cytology/drug effects;
CD8-Positive T-Lymphocytes/*immunology;
Carrier Proteins/metabolism;
Cell Differentiation/drug effects;
Cell Nucleus/drug effects/metabolism;
Cell Proliferation/drug effects;
Cytokines/biosynthesis;
Dendritic Cells/cytology/drug effects/enzymology/*immunology;
Enzyme Activation/drug effects;
Lymphocyte Activation/*drug effects;
Membrane Glycoproteins/metabolism;
Mice;
Mice, Inbred C57BL;
Mice, Knockout;
Mitogen-Activated Protein Kinases/metabolism;
Myeloid Differentiation Factor 88/metabolism;
NF-kappa B/metabolism;
Neoplasms/immunology/*pathology;
Pectins/*pharmacology;
Phenotype;
Protein Transport/drug effects;
Receptors, Chemokine/metabolism;
Signal Transduction/drug effects;
T-Lymphocytes, Cytotoxic/cytology/drug effects;
Toll-Like Receptor 4/*agonists/metabolism
- From:Experimental & Molecular Medicine
2013;45(2):e8-
- CountryRepublic of Korea
- Language:English
-
Abstract:
We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1beta, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8+ T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway.
