Flow Cytometric DNA Content Analysis in Breast Cancer Comparison study of fresh and paraffin-embedded tissues.
- Author:
Jin Ye YOO
;
Hye Jae CHO
- Publication Type:Original Article
- Keywords:
Flow cytometry;
Ploidy;
S-phase fraction;
Breast carcinoma
- MeSH:
Aneuploidy;
Breast Neoplasms*;
Breast*;
DNA*;
Flow Cytometry;
Ploidies
- From:Korean Journal of Pathology
1998;32(11):993-999
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
DNA content of 25 cases of breast carcinoma was analyzed by flow cytometry in both fresh and formalin-fixed, paraffin-embedded tissue. Aneuploidy in fresh tissue and paraffin-embedded tissues was 72% and 32%, respectively. There was a 52% agreement in analysis of DNA ploidy between fresh and paraffin-embedded tissues. Most of the discrepancies resulted from loss of aneuploid peaks on the histograms of paraffin-embedded tissue. Mean S-phase fraction was slightly higher in a paraffin-embedded tissue than that in the fresh tissue; 19.2 9.1% versus 16.1 8.8% and there was no significant correlation between the S-phase fractions. In statistical analysis, the histologic and nuclear grades were not correlated with ploidy or mean S-phase fraction. Therefore it is strongly recommended to use the fresh tissue in flow cytometric DNA content analysis of breast cancer.
