Characteristics of Intron 6 Nucleotide Sequences of cis-AB in Koreans.
- Author:
Ha Nui KIM
1
;
Hye Jin LEE
;
Gye Ryung CHOI
;
Myung Han KIM
;
Jang Su KIM
;
Chae Seung LIM
;
Young Kee KIM
;
Kap No LEE
Author Information
1. Department of Laboratory Medicine, School of Medicine, Korea University, Seoul, Korea. malarim@korea.ac.kr
- Publication Type:Original Article
- Keywords:
cis-AB;
Blood typing;
ABO blood group;
Glycosyltransferase;
Intron 6
- MeSH:
ABO Blood-Group System;
Alleles;
Base Sequence;
Blood Grouping and Crossmatching;
Clone Cells;
Cloning, Organism;
Exons;
Introns;
Nucleotides;
Phenotype;
Polymorphism, Single Nucleotide;
Serologic Tests
- From:Korean Journal of Blood Transfusion
2010;21(3):230-235
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The cis-AB is a very rare phenotype in the ABO blood group system. It corresponds to a special ABO allele that encodes glycosyltransferase that is capable of synthesizing both A and B antigens. Until now, the exon 6 and 7 gene sequences of cis-AB alleles are well known. In this study, we report on the intron 6 sequence structure of the cis-AB allele. METHODS: Standard serologic tests for the ABO blood group phenotypes were performed in four cis-AB samples. Allele-separation by cloning and subsequent sequencing was carried out. RESULTS: The results showed that intron 6 of cis-AB is almost identical to the A101 allele except for three single nucleotide polymorphisms at nucleotide positions 163, 179 and 662, where the nucleotides of the A101 replace those of B101. CONCLUSION: The intron 6 sequences of cis-AB in Koreans have both A101 and B101 blood group sequences.
