Confirmation of HCV Positivity for Indeterminate Donors in Anti-HCV Antibody Immunoblot Assay among Blood Donors.
- Author:
Youn Jung CHO
1
;
Dong Hee SEO
;
Byung Gap HWANG
;
Dong Jip KIM
;
Nam Hyun CHUNG
Author Information
1. Blood Transfusion Research Institute, The Republic of Korea National Red Cross, Korea.
- Publication Type:Original Article
- Keywords:
RIBA;
Indeterminate result;
HCV;
blood donor
- MeSH:
Blood Donors*;
Humans;
Immunoenzyme Techniques;
Red Cross;
RNA;
Tissue Donors*
- From:Korean Journal of Blood Transfusion
2001;12(2):237-244
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In Korean Red Cross, recombinant immunoblot assay (RIBA) has been used for the confirmatory test of HCV positive units since 1995. To certify the HCV infection in blood donors who showed the 'indeterminate result on the RIBA test, this study was performed. METHODS: Three enzyme immunoassay (EIA) kits (LG HCD 3.0, DONG-A HCV 3.0, and ORTHO HCV 3.0)and RNA detection method were employed to evaluate infection state of 135 samples of the 'indeterminate in the RIBA test. RESULTS: The 52.6% of the samples showed the same test results with three EIA kits. Fifteen samples (11.1%) were HCV RNA positive with RT-PCR-hybridization technique. Among 15 samples of HCV RNA positive, 13 (86.7%), 13 (86.7%), and 14(93.3%) of samples were positive in LG HCD 3.0, Ortho HCV 3.0 and Dong-A HCV 3.0 EIA, respectively. In the analysis of RIBA band reaction, HCV RNA positivity were correlated with core14, core518, and 897 antigen. However, among 64 samples which react with core antigen only, five samples (7.8%) were HCV RNA positive. CONCLUSION: Based on the results of the present study, it is recommend that the HCV RNA test be used as a method of confirmatory test in order to notify exact HCV positivity status to blood donor who showed indeterminate RIBA result.
