Identification of Candida Species by Analysis of rRNA Spacer Regions.
- Author:
Mi Kyung LEE
1
;
Hye Ryoun KIM
;
Byung In RO
Author Information
1. Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea. cpworld@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Candida;
tRNA intergenic length polymorphism (tDNA-ILP);
Species identification
- MeSH:
Candida*;
Electrophoresis;
Gels;
Polymerase Chain Reaction;
RNA, Transfer;
Sepharose
- From:Korean Journal of Medical Mycology
2006;11(1):13-18
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The genus Candida comprises 163 species, the most common pathogen in the genus is C. albicans, however, other Candida species are considered as emergent pathogens. Because there are species-specific differences in the susceptibility of Candida spp. to the currently used therapeutic drugs, species identification is critical for therapeutic planning and accurate epidemiological records. The objective of this study is to evaluate the performance of tRNA intergenic length polymorphism (tDNA-ILP) analysis for the accurate identification of Candida species. METHODS: For the identifying the 7 type strains and 54 clinical isolates of Candida, the suitability of tDNA-ILP was evaluated. The polymerase chain reaction (PCR) using a pair of primers or a single primer was performed. The PCR products were separated by electrophoresis in 2% agarose gels for 70 min. RESULTS: In seven Candida type strains, tDNA-ILP using a single primer (reverse) can be easily analyzed by visual comparision. Fifty-three of 54 strains were identified as the same species with conventional identification. CONCLUSIONS: The tDNA-ILP analysis can be useful for the simple and rapid identification of Candida species in routine laboratories.
