Analysis of Pretransplant ELISA-Panel Reactive Antibody in Kidney Transplant Patients.
- Author:
Eun Jee OH
1
;
Yeon Joon PARK
;
Kyo Young LEE
;
Bum Soon CHOI
;
Chul Woo YANG
;
Yong Soo KIM
;
Byung Kee BANG
;
Yong Bok KOH
;
Chang Suk KANG
Author Information
1. Department of Clinical Pathology, The Catholic University of Korea, Seoul, Korea. ejoh@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Kidney;
Transplantation;
Panel reactive antibody (PRA);
ELISA;
Rejection;
Crossmatch
- MeSH:
Allografts;
Antibodies;
Enzyme-Linked Immunosorbent Assay;
Female;
Humans;
Incidence;
Kidney*;
Living Donors;
Male;
Pregnancy;
Transplantation;
Transplants
- From:The Journal of the Korean Society for Transplantation
2004;18(2):134-139
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: HLA antibodies have been shown to be associated with graft loss of organ transplants in prior studies. This study was designed to analyze the results of ELISA- panel reactive antibody (ELISA-PRA) in kidney transplant patients and the impact of this test on the clinical outcome. METHODS: We have investigated ELISA-PRA results from 110 living donor renal transplant patients from Nov. 2001 to Apr. 2004. RESULTS: ELISA-PRA positivity was found in 22 (20%) patients and was higher in the female patients than male (P<0.05). Pretransplant transfusion, pregnancy or transplantation history was not significantly correlated with ELISA- PRA result. ELISA-PRA (+)patients had more rejection episodes of 41% (n=9) (P=0.0005) and graft failures of 18% (n=4) (P=0.028) than ELISA-PRA (-), which had 8% (n=7) and 3% (n=3), respectively. Patients group with a result of ELISA-PRA/flowcytometric crossmatch (FCXM) (+/ -) or (+ / +) had worse clinical outcome than ELISA- PRA/FCXM ( -/- ). ELISA-PRA/FCXM (+ /+ ) correlated with higher incidence of allograft rejection than ELIS- PRA/FCXM (+ /- ) or ( -/+ ). CONCLUSION: These results suggest that in conjunction with FCXM results, pretransplant ELISA-PRA test is useful predictor of clinical outcome in renal transplant recipients.
