Fucodiphlorethol G Purified from Ecklonia cava Suppresses Ultraviolet B Radiation-Induced Oxidative Stress and Cellular Damage.
- Author:
Ki Cheon KIM
1
;
Mei Jing PIAO
;
Jian ZHENG
;
Cheng Wen YAO
;
Ji Won CHA
;
Madduma Hewage Susara Ruwan KUMARA
;
Xia HAN
;
Hee Kyoung KANG
;
Nam Ho LEE
;
Jin Won HYUN
Author Information
1. School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea. jinwonh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Fucodiphlorethol G;
Ultraviolet B;
Mitochondria membrane potential;
Reactive oxygen species;
Human keratinocytes
- MeSH:
Apoptosis;
Caspase 9;
DNA Fragmentation;
Humans;
Keratinocytes;
Membrane Potential, Mitochondrial;
Oxidative Stress*;
Reactive Oxygen Species
- From:Biomolecules & Therapeutics
2014;22(4):301-307
- CountryRepublic of Korea
- Language:English
-
Abstract:
Fucodiphlorethol G (6'-[2,4-dihydroxy-6-(2,4,6-trihydroxyphenoxy)phenoxy]biphenyl-2,2',4,4',6-pentol) is a compound purified from Ecklonia cava, a brown alga that is widely distributed offshore of Jeju Island. This study investigated the protective effects of fucodiphlorethol G against oxidative damage-mediated apoptosis induced by ultraviolet B (UVB) irradiation. Fucodiphlorethol G attenuated the generation of 2, 2-diphenyl-1-picrylhydrazyl radicals and intracellular reactive oxygen species in response to UVB irradiation. Fucodiphlorethol G suppressed the inhibition of human keratinocyte growth by UVB irradiation. Additionally, the wavelength of light absorbed by fucodiphlorethol G was close to the UVB spectrum. Fucodiphlorethol G reduced UVB radiation-induced 8-isoprostane generation and DNA fragmentation in human keratinocytes. Moreover, fucodiphlorethol G reduced UVB radiation-induced loss of mitochondrial membrane potential, generation of apoptotic cells, and active caspase-9 expression. Taken together, fucodiphlorethol G protected human keratinocytes against UVB radiation-induced cell damage and apoptosis by absorbing UVB radiation and scavenging reactive oxygen species.
