Dermal Stability and In Vitro Skin Permeation of Collagen Pentapeptides (KTTKS and palmitoyl-KTTKS).
- Author:
Yun Lim CHOI
1
;
Eun Ji PARK
;
Eunje KIM
;
Dong Hee NA
;
Young Hee SHIN
Author Information
1. College of Pharmacy, Kyungsung University, Busan 608-736, Republic of Korea. yhshin@ks.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Collagen pentapeptide;
KTTKS;
Palmitoyl-KTTKS;
Stability;
Permeability
- MeSH:
Animals;
Collagen*;
Dermis;
Epidermis;
Mice;
Mice, Hairless;
Permeability;
Protease Inhibitors;
Skin*
- From:Biomolecules & Therapeutics
2014;22(4):321-327
- CountryRepublic of Korea
- Language:English
-
Abstract:
Collagen pentapeptide (Lys-Thr-Thr-Lys-Ser, KTTKS) and its palmitoylated derivative (pal-KTTKS) have received a great deal of attention as cosmeceutical ingredients for their anti-wrinkle effects. The objective of this study was to evaluate stability and permeability of KTTKS and pal-KTTKS in hairless mouse skin. In this study, a liquid chromatography-tandem mass spectrometric method was developed for the quantification of pal-KTTKS, and used for stability and permeability studies. Stability studies were performed using skin extracts and homogenates. Both KTTKS and pal-KTTKS were rapidly degraded, but pal-KTTKS was more stable than KTTKS. When protease inhibitors were added, the stability of both compounds (KTTKS and pal-KTTKS) improved significantly. In the skin permeation study, neither KTTKS nor pal-KTTKS was detected in the receptor solution, which indicates that neither compound could permeate through the full-thickness hairless mouse skin in the experimental conditions of this study. While KTTKS was not detected in any of the skin layers (the stratum corneum, epidermis, and dermis), pal-KTTKS was observed in all skin layers: 4.2 +/- 0.7 microg/cm2 in the stratum corneum, 2.8 +/- 0.5 microg/cm2 in the epidermis, and 0.3 +/- 0.1 microg/cm2 in the dermis. In conclusion, this study indicated that pal-KTTKS had greater stability and permeability than that of un-modified KTTKS, and may be a useful anti-wrinkle and anti-aging cosmeceutical agent.
