Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana.
- Author:
Wei LI
1
;
Xi Tao YAN
;
Ya Nan SUN
;
Thi Thanh NGAN
;
Sang Hee SHIM
;
Young Ho KIM
Author Information
1. School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea. shshim29@ynu.ac.kr
- Publication Type:Original Article
- Keywords:
Pulsatilla koreana;
NF-kappaB inhibitory activity;
Tumor necrosis factor-alpha;
PPAR
- MeSH:
Anti-Inflammatory Agents;
Hep G2 Cells;
Inhibitory Concentration 50;
Intercellular Adhesion Molecule-1;
Luciferases;
Methanol;
NF-kappa B;
Peroxisome Proliferator-Activated Receptors*;
Pulsatilla*;
RNA, Messenger;
Saponins*;
Tumor Necrosis Factor-alpha
- From:Biomolecules & Therapeutics
2014;22(4):334-340
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-kappaB inhibitory activity of the isolated compounds was measured in TNFalpha-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNFalpha-stimulated NF-kappaB activation in a dose-dependent manner, with IC50 values ranging from 0.75-8.30 microM. Compounds 19 and 20 also inhibited the TNFalpha-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9-10.8 microM. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.
