Human Umbilical Cord Blood-derived Cells Generate Insulin-producing Cells In Vitro.
- Author:
Hee Jeong CHEONG
1
;
Sook Ja KIM
;
Mi Sun LIM
;
Jong Ho WON
;
Hyeong Kyu PARK
;
Dongho CHOI
Author Information
1. Stem Cell Therapy Center, Soonchunhyang University Hospital, Korea. dhchoi@hosp.sch.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Human umbilical cord blood (hUCB);
Insulin-producing cells (IPCs);
Islet
- MeSH:
C-Peptide;
Cell Count;
Collagen Type I;
Dimethyl Sulfoxide;
Fetal Blood;
Humans*;
Immunohistochemistry;
Insulin;
Islets of Langerhans;
Plastics;
Stem Cells;
Umbilical Cord*
- From:The Journal of the Korean Society for Transplantation
2007;21(1):31-37
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Here we showed that human umbilical cord blood (hUCB)-derived cells, when cultured under defined conditions, generated insulin-producing cells (IPCs). METHODS: hUCB mononuclear cells (MNCs) were cultured in serum-free low (5.5 mM glucose) DMEM at a cell density of 3x10(6)/cm2 in the presence of 1% DMSO for 3 days followed by high (25 mM glucose) DMEM supplemented with 10% FBS for 7 additional days. They were plated in plastic six well plates on slide coverslips (22x22 mm2) coated with 0.006% type I collagen. RESULTS: These IPCs formed clusters similar to islets of Langerhans. We confirmed these clusters were positive for insulin and C-peptide by immunohistochemistry. CONCLUSION: Our data demonstrated that in vitro hUCB-derived cells generated IPCs, which can be a potential source for the treatment of diabetes via a stem cell therapy approach.
