Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs.
10.3347/kjp.2002.40.1.59
- Author:
Dirk C DE GRAAF
;
Hans DE CONINCK
;
Franz PETRY
;
Ilka B EECKHOUT
;
Johan E PEETERS
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Cryptosporidium;
Antigen;
Gene cloning;
Zinc-finger;
Bovine
- MeSH:
Amino Acid Sequence;
Animals;
Antibodies, Protozoan/*blood;
Antigens, Protozoan/chemistry/*immunology;
Base Sequence;
Cattle/*immunology;
Cryptosporidium parvum/*immunology;
Molecular Sequence Data;
Protozoan Proteins/chemistry/genetics;
Rabbits;
Recombinant Proteins;
Zinc Fingers/genetics/*immunology
- From:The Korean Journal of Parasitology
2002;40(1):59-64
- CountryRepublic of Korea
- Language:English
-
Abstract:
A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
