Effect of Creatine on the Survival of RGC-5 Cells under Serum Deprivation.
10.3341/jkos.2011.52.5.618
- Author:
Jae Woo KIM
1
;
Jung Heum HONG
;
Sun Hee KANG
;
Yun Young KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Creatine;
Cytoprotection;
Retinal ganglion cells
- MeSH:
Apoptosis;
Creatine;
Culture Media, Serum-Free;
Cytoprotection;
Oxazines;
Retinal Ganglion Cells;
Xanthenes
- From:Journal of the Korean Ophthalmological Society
2011;52(5):618-623
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the protective effect of creatine on the survival of retinal ganglion cells after serum deprivation. METHODS: RGC-5 cells were exposed to 5 mM creatine with serum-free media for 4 days. Cellular survival and mitochondrial respiratory activity were measured with MTT assay and resazurin assay, respectively. Degree of apoptosis was evaluated with vital staining using acridine orange/Hoechest 33342 and flow cytometric analysis using annexin/PI, respectively. RESULTS: Creatine increased cellular survival of RGC-5 cells significantly after serum deprivation. Additionally, creatine increased mitochondrial respiratory activity and inhibited apoptosis of RGC-5 cells. CONCLUSIONS: The energy precursor creatine increased survival of retinal ganglion cells after serum deprivation. Creatine could be relevant for the cytoprotection of retinal ganglion cells.
