The absolute number of CD34+ cells predicts optimal timing of progenitor cell collection and posttransplant hematopoietic recovery.
- Author:
Mi Ae LEE
1
;
Seok LEE
;
Chu Myong SEONG
;
Wha Soon CHUNG
Author Information
1. Department of Clinical Pathology, Ewha Womans University Mokdong Hospital, Seoul, Korea. miae@mm.ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Peripheral blood progenitor cell(PBPC);
Absolute number of CD34+ cells;
Posttransplant hematopoietic recovery;
Peripheral blood progenitor cell transplantation(PBPCT)
- MeSH:
Cell Count;
Granulocyte Colony-Stimulating Factor;
Humans;
Leukapheresis;
Leukocyte Count;
Stem Cells*;
Tissue Donors
- From:Korean Journal of Clinical Pathology
2000;20(1):103-109
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recently, the commercial kits for measurement of the absolute number of CD34+ cells have been introduced as a standard method. The aims of this study was to investigated optimal timing of peripheral blood progenitor cell(PBPC) collection and optimal CD34+ cells dose transplanted by measurement of the absolute CD34+ cells. METHODS: We measured total leukocyte count, mononuclear cell count and the absolute number of CD34+ cells using ProCOUNT(Becton Dickinson, USA) in peripheral blood from 54 patients and 7 normal donors who underwent 101 leukapheresis for PBPC collection. We studied correlations among the absolute number of circulating CD34+ cells, other predictors and harvesting yields. We investigated relationships between the posttransplant hematopoietic recovery and CD34+ cells dose in 30 patients. RESULTS: The total number of CD34+ cells in harvesting products could be mostly predicted from the absolute number of circulating CD34+ cells. From 4 to 6 day after G-CSF mobilization, the absolute number of circulating CD34+ cells was peaked. A number of circulating CD34+ cells more than 20/microliter ensured 2.5x106 CD34+ cells/Kg in harvesting products. The patients received CD34+ cells dose >3.5x106/Kg led to a significantly faster recovery of platelets, compared with the patients receiving <3.5x106 CD34+ cells/Kg(P<0.05). CONCLUSIONS: These results suggest that PBPC collection should be started at day of circulating CD34+ cells more than 20/microliter or 4-6 days after G-CSF mobilization for successful leukapheresis and the CD34+ cell dose more than 3.5x106/Kg for PBPC transplantation could predicted rapid hematopoietic recovery.
