The Study of CYFRA 21-1 and Epidermal Growth Factor Receptor Levels in Cancer Tissue of Bronchogenic Carcinoma Patients.
- Author:
Dae Yun KIM
1
;
Song Myung KIM
Author Information
1. Department of Thoracic and Cardiovascular Surgery, College of Medicine, Kosin University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Lung neoplasm;
Neoplasm marker
- MeSH:
Adenocarcinoma;
Antibodies, Monoclonal;
Carcinoma, Bronchogenic*;
Carcinoma, Squamous Cell;
Cell Membrane;
Cytoplasm;
Epidermal Growth Factor*;
Humans;
Keratin-19;
Lung;
Lung Neoplasms;
Protein-Tyrosine Kinases;
Receptor, Epidermal Growth Factor*;
Receptors, Growth Factor;
Skeleton;
Transforming Growth Factor alpha
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
1997;30(9):854-861
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
CYFRA 21-1 is known to be a cytokeratin 19 fragment, and it can be detected by using two specific monoclonal antibodies (KS 19-1 and BM 19-21) and can be clinically applied as a useful circulating tumor marker. The epidermal growth factor receptor (EGF-R) expression was evaluated and characterized by its tyrosine protein kinase activity and by its ligand-stimulated autophosphorylation, a property shared with other peptide growth factor receptors. Autocrine or paracrine action was initiated by a growth factor, or by a transforming growth factor alpha, which had an extensive homology with EGF and which also stimulated tyrosine kinase activity on the EGF-R. The CYFRA 21-1 and the EGF-R levels in 30 patients with primary lung tumors were investigated. There were 24 patients with squamous cell carcinomas and 6 patients with adenocarcinomas. Specimen 5 mm3 in size were sampled at three different locations ; the main lesion, the boundary between the lesion and the unaffected tissue, and the unaffected tissue of the patients. The results were as follows : 1. The CYFRA 21-1 concentration in the cancer boundary, the most malignant region,(348.6+/-89.9 ng/ml) was the lowest value. The CYFRA 21-1 concentration in unaffected tissue,(718.4+/-77.8 ng/ml) was higher than that in the main lesion. which had intact cellularity. 2. The EGF-R concentration in the main lesion was higher than that in the unaffected tissue, and the EGF-R concentration in a squamous cell cacinoma was higher than that in an adenocarcinoma. also, the EGF-R concentration in the cancer boundary was highest at stage I, II. The EGF-R concentration was higher in the main cancer lesion that in the unaffected tissue at stage III, IV. 3. The CYFRA 21-1 was a cytoplasmic skeleton and the EGF-R was a cell-wall component; there was no correlation. In conclusion, CYFRA 21-1 was abundant in the cytoplasm but had a higher concentration in the unaffected tissue than in the main cancer lesion. The CYFRA 21-1 concentration of the tissue did not reflect the amount of cancer activity, the EGF-R was located in the cell membrane, the level of tissue that reflects cancer activity, so the main cancer lesion had a higher concentration than the unaffected tissue. CYFRA 21-1 is not a useful tumor maker at the tissue level. Because the EGF-R concentration reflected the cancer activity, its a useful tumor marker for lung cancer.
