Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy.
- Author:
Yu Hsiang HSU
1
;
Hsing Hui LI
;
Junne Ming SUNG
;
Wei Yu CHEN
;
Ya Chin HOU
;
Yun Han WENG
;
Wei Ting LAI
;
Chih Hsing WU
;
Ming Shi CHANG
Author Information
- Publication Type:Original Article
- MeSH: Animals; Apoptosis; Blood Glucose; Caspase 8; Diabetes Mellitus; Diabetic Nephropathies*; Enzyme-Linked Immunosorbent Assay; Glucose; Humans; Hydrogen Peroxide; In Vitro Techniques; Interleukin-10; Interleukins; Kidney; Kidney Failure, Chronic; Mice; Podocytes*; Rats; Vascular Endothelial Growth Factor A
- From:Experimental & Molecular Medicine 2017;49(3):e310-
- CountryRepublic of Korea
- Language:English
- Abstract: Interleukin (IL)-20, a proinflammatory cytokine of the IL-10 family, is involved in acute and chronic renal failure. The aim of this study was to elucidate the role of IL-20 during diabetic nephropathy development. We found that IL-20 and its receptor IL-20R1 were upregulated in the kidneys of mice and rats with STZ-induced diabetes. In vitro, IL-20 induced MMP-9, MCP-1, TGF-β1 and VEGF expression in podocytes. IL-20 was upregulated by hydrogen peroxide, high-dose glucose and TGF-β1. In addition, IL-20 induced apoptosis in podocytes by activating caspase-8. In STZ-induced early diabetic nephropathy, IL-20R1-deficient mice had lower blood glucose and serum BUN levels and a smaller glomerular area than did wild-type controls. Anti-IL-20 monoclonal antibody (7E) treatment reduced blood glucose and the glomerular area and improved renal functions in mice in the early stage of STZ-induced diabetic nephropathy. ELISA showed that the serum IL-20 level was higher in patients with diabetes mellitus than in healthy controls. The findings of this study suggest that IL-20 induces cell apoptosis of podocytes and plays a role in the pathogenesis of early diabetic nephropathy.
