From rabbit antibody repertoires to rabbit monoclonal antibodies.
- Author:
Justus WEBER
1
;
Haiyong PENG
;
Christoph RADER
Author Information
- Publication Type:Review
- MeSH: Antibodies, Monoclonal*; B-Lymphocytes; Bacteriophages; Humans; Hybridomas; Indicators and Reagents; Sensitivity and Specificity
- From:Experimental & Molecular Medicine 2017;49(3):e305-
- CountryRepublic of Korea
- Language:English
- Abstract: In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
