The Stem Bark of Kalopanax pictus Exhibits Anti-inflammatory Effect through Heme Oxygenase-1 Induction and NF-kappaB Suppression.
- Author:
Soo Young BANG
1
;
Ga Young PARK
;
Sun Young PARK
;
Ji Hee KIM
;
Yun Kyoung LEE
;
Sang Joon LEE
;
YoungHee KIM
Author Information
- Publication Type:Original Article
- Keywords: Kalopanax pictus; NO; NF-kappaB; MAPK; HO-1; Nrf2
- MeSH: Animals; Arthritis; Cell Survival; China; Diabetes Mellitus; Heme; Heme Oxygenase-1; I-kappa B Proteins; JNK Mitogen-Activated Protein Kinases; Kalopanax; Korea; Macrophages; Medicine, Traditional; Mice; NF-kappa B; Nitric Oxide Synthase; Phosphorylation; Phosphotransferases
- From:Immune Network 2010;10(6):212-218
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The stem bark of Kalopanax pictus (KP) has been used in traditional medicine to treat rheumatoidal arthritis, neurotic pain and diabetes mellitus in China and Korea. In this study, the mechanism responsible for anti-inflammatory effects of KP was investigated. METHODS: We examined the effects of KP on NO production, nitric oxide synthase (iNOS) and HO-1 expression, NF-kappaB, Nrf2 and MAPK activation in mouse peritoneal macrophages. RESULTS: The aqueous extract of KP inhibited LPS-induced NO secretion as well as inducible iNOS expression, without affecting cell viability. KP suppressed LPS-induced NF-kappaB activation, phosphorylation and degradation of IkappaB-alpha, phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). Furthermore, KP induced HO-1 expression and Nrf2 nuclear translocation. CONCLUSION: These results suggest that KP has the inhibitory effects on LPS-induced NO production in macrophages through NF-kappaB suppression and HO-1 induction.
