Mutations in folP1, rpoB, and gyrB associated with multiple drug resistance in Mycobacterium leprae isolates from South Korea.
- Author:
Seong Beom LEE
1
;
Eun Young YOU
;
Tae Jin KANG
;
Se Kon KIM
;
Jong Pill KIM
;
Young Hoon KO
;
Gue Tae CHAE
Author Information
1. Institute of Hansen's disease, Department of Pathology, College of Medicine, The Catholic University of Korea, Korea.
- Publication Type:Original Article
- Keywords:
Multiple drug resistance;
mutation;
folP1;
rpoB;
gyrB
- MeSH:
Dapsone;
Drug Resistance, Multiple*;
Humans;
Korea*;
Leprosy;
Mutation, Missense;
Mycobacterium leprae*;
Mycobacterium*;
Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:Korean Leprosy Bulletin
2003;36(1):3-9
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The emergence of multiple drug resistant Mycobacterium leprae has emphasized the need for early decision of effective antileprosy drug in the treatment for leprosy patients. Mutations in the genes associated with multiple drug resistance in Mycobacterium leprae isolates from 17 South Korean patients, who were already confirmed to have mutations in folP1 gene, were investigated using a PCR - single strand conformation polymorphism (SSCP) - DNA sequencing assay. Two strains, which has double mutations, were found in the two unrelated patients : one missense mutation in folP1 (Arg 55 for Pro) and in rpoB (Gly 522 for Ser), and in folP1 (Ala 53 for Thr) and in gyrB (Asn 426 for Asp), respectively. The patients were treated with the long monotheraphy of dapsone or with the inappropriate regimen of antileprosy drugs. These results emphasize the importance of multi-drug theraphy in order to prevent mult-idrug resistance and assist in the choice of the appropriate regimens for treating leprosy.
