Optimization of Extraction Conditions for Active Compounds of Herbal Medicinal Formula, DF, by Response Surface Methodology.
- Author:
Birang JEONG
1
;
Seong Yeon CHOI
;
Hyeon Seok JANG
;
Guijae YOO
;
Seung Hyun KIM
;
Jung Hwan KIM
;
Yong Soo KWON
;
Jong Seong ROH
;
Yoosik YOON
;
Soon Shik SHIN
;
Heejung YANG
Author Information
- Publication Type:Original Article
- Keywords: Response surface methodology; Ephedra intermedia; (−)-ephedrine; (+)-pseudoephedrine; HPLC
- MeSH: Chromatography, High Pressure Liquid; Ephedra; Korea; Lithospermum; Metabolic Diseases; Obesity; Rheum
- From:Natural Product Sciences 2017;23(1):9-15
- CountryRepublic of Korea
- Language:English
- Abstract: DF formula is comprised of three traditional herbs, Ephedra intermedia, Rheum palmatum and Lithospermum erythrorhizon, and locally used for treating of the metabolic diseases, such as obesity and diabetes in Korea. We tried to optimize the extraction conditions of two major components, (−)-ephedrine and (+)-pseudoephedrine, in DF formula using response surface methodology with Box-Behnken design (BBD). The experimental conditions with 70% for EtOH concentrations, 4.8 hour for extraction hours and 8.7 times for the solvent to material ratio were suggested for the optimized extraction of DF formula with the highest amounts of (−)-ephedrine and (+)-pseudoephedrine in the designed model.
