Diarylbutane-type Lignans from Myristica fragrans (Nutmeg) show the Cytotoxicity against Breast Cancer Cells through Activation of AMP-activated Protein Kinase.
10.20307/nps.2017.23.1.21
- Author:
Thi Van Thu LE
1
;
Phi Hung NGUYEN
;
Hong Seok CHOI
;
Jun Li YANG
;
Keon Wook KANG
;
Sang Gun AHN
;
Won Keun OH
Author Information
1. College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Myristica fragrans;
Diarylbutane lignan;
MCF-7;
AMP-activated protein kinase (AMPK)
- MeSH:
Agar;
AMP-Activated Protein Kinases*;
Apoptosis;
Breast Neoplasms*;
Breast*;
Humans;
Injections, Intraperitoneal;
Lignans*;
MCF-7 Cells;
Myristica fragrans*;
Phosphorylation
- From:Natural Product Sciences
2017;23(1):21-28
- CountryRepublic of Korea
- Language:English
-
Abstract:
In our program to search for new AMP-activated protein kinase (AMPK) activators from plants that exert potential anticancer property, we found that an EtOAc extract of Myristica fragrans (nutmeg) activated AMPK enzyme in human breast cancer MCF-7 cells. Two major diarylbutane-type lignans, macelignan and meso-dihydroguaiaretic acid (MDGA), were isolated as active principles from this extract. Treatment of breast cancer cells with two compounds induced cellular apoptosis, evidenced by cleavage of poly-(ADP-ribose) polymerase (PARP) and Ser 15 phosphorylation of p53. Moreover, macelignan and MDGA significantly inhibited the colony formation of MCF-7 breast cancer cells on soft agar. Intraperitoneal injection of macelignan and MDGA (20 mg/kg) suppressed the tumor growth of 4T1 mammary cancer cells. These results indicate that the chemopreventive effects of two major diarylbutane-type lignans from Myristica fragrans (nutmeg) may be associated with induction of apoptosis presumably through AMPK activation.
