Comprehensive Evaluation of the Anti-Helicobacter pylori Activity of Scutellariae Radix.
10.20307/nps.2017.23.1.46
- Author:
Ba Wool LEE
1
;
Il Ho PARK
;
Dongsool YIM
;
Sung Sook CHOI
Author Information
1. College of Pharmacy, Sahmyook University, 815, Hwarang-ro, Nowon-gu, Seoul, 01795 Republic of Korea. sschoi@syu.ac.kr
- Publication Type:Original Article
- Keywords:
Scutellariae Radix;
Helicobacter pylori;
MIC;
DNA protection assay;
Urease inhibitory activity
- MeSH:
DNA;
Helicobacter pylori;
Microbial Sensitivity Tests;
Real-Time Polymerase Chain Reaction;
Scutellaria baicalensis*;
Scutellaria*;
Urease
- From:Natural Product Sciences
2017;23(1):46-52
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to evaluate the anti-Helicobacter pylori activity of fractions and major aglycon compounds (baicalein, chrysin, oroxylin A, wogonin) of Scutellariae Radix. Minimum inhibitory concentration (MIC) measurement, DPPH radical-scavenging assay, DNA protection assay, and urease inhibition analysis were performed. The ethyl acetate (EtOAc) fraction showed the potent anti-Helicobacter activity, and therefore, compounds in the EtOAc fraction were subjected to further assay. The MICs of chrysin, oroxylin A, and wogonin against Helicobacter pylori 26695 were 6.25, 12.5 and 25 µg/mL, respectively. Baicalein exhibited the most effective DPPH radical-scavenging activity. DNA protection using Fenton reaction, chrysin, oroxylin A, and wogonin showed effective DNA protective effect. This result was also confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Regarding Jack bean urease (0.5 mg/mL, 50 unit/mg) inhibition, 20 mM ofbaicalein and chrysin inhibited urease activity by 88.2% and 72.5%, respectively.
