Ethanol Extract of Perillae Herba Enhances Pentobarbital-Induced Sleep and Non-Rapid Eye Movement (NREM) Sleep through GABA(A)-ergic Systems.
10.20307/nps.2017.23.1.53
- Author:
Yeong Ok KWON
1
;
Tae Woo HA
;
Ki Wan OH
Author Information
1. College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea. kiwan@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Perillae Herba ethanol extract (PHEE);
Pentobarbital-induced sleep;
Glutamic acid decarboxylase (GAD);
GABA;
GABAA receptors;
Electroencephalogram (EEG)
- MeSH:
Animals;
Electroencephalography;
Ethanol*;
Eye Movements*;
gamma-Aminobutyric Acid;
Glutamate Decarboxylase;
Hypothalamus;
Mice;
Motor Activity;
Pentobarbital;
Perilla*;
Rats;
Receptors, GABA;
Wakefulness
- From:Natural Product Sciences
2017;23(1):53-60
- CountryRepublic of Korea
- Language:English
-
Abstract:
Perillae Herba has been traditionally used for the sedation in the oriental countries. Therefore, this study was conducted to determine whether Perillae Herba ethanol extract (PHEE) enhances pentobarbital-induced sleeping behaviors in animals. In addition, the possible mechanisms are demonstrated. PHEE (12.5, 25 and 50 mg/kg. p.o.) reduced the locomotor activity in mice. PHEE reduced sleep latency and augmented the total sleep time in pentobarbital (42 mg/kg, i.p.)-induced sleep in mice. Furthermore, the number of sleeping mice treated with sub-hypnotic pentobarbital (28 mg/kg, i.p.) increased. PHEE (50 mg/kg. p.o.) decreased the sleep/wake cycles and wakefulness, and increased total sleeping time and NREM sleep in electroencephalogram (EEG) of rats. In addition, PHEE (0.1, 1.0 and 10 µg/ml) increased the intracellular Cl⁻ level through the GABA receptors in the hypothalamus of rats. Moreover, the protein of glutamate decarboxylase (GAD) was overexpressed by PFEE. It was found that PHEE enhanced pentobarbital-induced sleeping behaviors through GABA(A)-ergic transmissions.
