Evaluation of Porcine Pancreatic Islets Transplanted in the Kidney Capsules of Diabetic Mice Using a Clinically Approved Superparamagnetic Iron Oxide (SPIO) and a 1.5T MR Scanner.
10.3348/kjr.2010.11.6.673
- Author:
Hoe Suk KIM
1
;
Hyoungsu KIM
;
Kyong Soo PARK
;
Woo Kyung MOON
Author Information
1. Department of Radiology, Seoul National University Hospital, Seoul 110-744, Korea. moonwk@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Porcine pancreatic islet;
Superparamagnetic iron oxide;
Magnetic resonance (MR);
Diabetes;
Xenotransplantation
- MeSH:
Animals;
Contrast Media/pharmacology;
Dextrans/pharmacology;
Diabetes Mellitus, Experimental/*therapy;
Glucose Tolerance Test;
*Islets of Langerhans Transplantation;
Magnetic Resonance Imaging/*methods;
Magnetite Nanoparticles;
Mice;
Microscopy, Electron;
Statistics, Nonparametric;
Swine
- From:Korean Journal of Radiology
2010;11(6):673-682
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To evaluate transplanted porcine pancreatic islets in the kidney capsules of diabetic mice using a clinically approved superparamagnetic iron oxide (SPIO) and a 1.5T MR scanner. MATERIALS AND METHODS: Various numbers of porcine pancreatic islets labeled with Resovist, a carboxydextran-coated SPIO, were transplanted into the kidney capsules of normal mice and imaged with a 3D FIESTA sequence using a 1.5T clinical MR scanner. Labeled (n = 3) and unlabeled (n = 2) islets were transplanted into the kidney capsules of streptozotocin-induced diabetic mice. Blood glucose levels and MR signal intensities were monitored for 30 days post-transplantation. RESULTS: There were no significant differences in viability or insulin secretion between labeled and unlabeled islets. A strong correlation (r2 > 0.94) was evident between the number of transplanted islets and T2 relaxation times quantified by MRI. Transplantation with labeled or unlabeled islets helped restore normal sustained glucose levels in diabetic mice, and nephrectomies induced the recurrence of diabetes. The MR signal intensity of labeled pancreatic islets decreased by 80% over 30 days. CONCLUSION: The transplantation of SPIO-labeled porcine islets into the kidney capsule of diabetic mice allows to restore normal glucose levels, and these islets can be visualized and quantified using a 1.5T clinical MR scanner.
