Enhancement of Antigen-specific Antibody and CD8(+) T Cell Responses by Codelivery of IL-12-encapsulated Microspheres in Protein and Peptide Vaccination.
- Author:
Su Hyung PARK
1
;
Jun CHANG
;
Se Hwan YANG
;
Hye Ju KIM
;
Hyun Hee KWAK
;
Byong Moon KIM
;
Sung Hee LEE
Author Information
- Publication Type:Original Article
- Keywords: Interleukin 12; PLGA microspheres; vaccine adjuvant; CTL response
- MeSH: Animals; Antibody Formation; Influenza, Human; Interleukin-12; Mice; Mice, Knockout; Microspheres*; Respiratory Syncytial Viruses; Vaccination*; Vaccines, Subunit
- From:Immune Network 2007;7(4):186-196
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Although IL-12 has been widely accepted to play a central role in the control of pathogen infection, the use of recombinant IL-12 (rIL-12) as a vaccine adjuvant has been known to be ineffective because of its rapid clearance in the body. METHODS: To investigate the effect of sustained release of IL-12 in vivo in the peptide and protein vaccination models, rIL-12 was encapsulated into poly (DL-lactic-co-glycolic acid) (PLGA). RESULTS: We found that codelivery of IL-12-encapsulated microspheres (IL-12EM) could dramatically increase not only antibody responses, but also antigen-specific CD4(+) and CD8(+) T cell responses. Enhanced immune responses were shown to be correlated with protective immunity against influenza and respiratory syncytial virus (RSV) virus challenge. Interestingly, the enhancement of CD8(+) T cell response was not detectable when CD4(+) T cell knockout mice were subjected to vaccination, indicating that the enhancement of the CD8(+) T cell response by IL-12EM is dependent on CD4(+) T cell "help". CONCLUSION: Thus, IL-12EM could be applied as an adjuvant of protein and peptide vaccines to enhance protective immunity against virus infection.
