Restriction Endonuclease Analysis of Plasmids and Antimicrobial Resistance Pattern of Staphylococcus Aureus and S. Epidermidis Isolated from Clinical Specimens.
- Author:
Hee Joo LEE
;
Jung Rim LEE
;
Mun Hee KIM
;
Jin Tae SUH
;
Young Il KIM
;
Whan Jo SUH
- Publication Type:Original Article
- MeSH:
Agar;
Bacteremia;
Coagulase;
Digestion;
DNA;
DNA Restriction Enzymes*;
Methicillin-Resistant Staphylococcus aureus;
Plasmids*;
Restriction Mapping;
Staphylococcus aureus*;
Staphylococcus*
- From:Korean Journal of Clinical Pathology
1997;17(2):252-259
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Methicillin-resistant Staphylococcus aureus is a major etiologic agent of hospital acquired infection. Coagulase negative staphylococci (CNS) species are previously regarded as contaminants. However nowadays CNS were regarded as an important cause of bacteremia. So in this study we wanted to analyze the patterns of plasmids and antimicrobial susceptibility test of Staphylococcus species isolated from clinical specimens. METHOD: Plasmid DNA was extracted and then processed through restriction enzyme digestion for plasmid analysis of S. aureus and antimicrobial susceptibility, which was done by agar dilution method. For S. epidermidis plasmid analysis was done without enzyme digestion. RESULTS: All of MRSA have 1 to 5 plasmids. There exists 6 patterns of S. aureus plasmid without enzyme digestion. With EcoRI and HindIII digestion pattern were more distinct and clear. For S. epidermidis enzyme digestion is not needed. Antimicrobial susceptibility patterns of S. aureus are simple whereas S. epidermidis showed variable patterns. CONCLUSIONS: For the plasmid analysis of S. aureus restriction enzyme digestion is required and for the S. epidermidis, the pattern of plasmids are variable so without restriction enzyme analysis we can obtain several patterns. Plasmid analysis will be used as a good epidemilogical tool for Staphylococcus.
