The inhibitory effect of lactic acid bacteria to periodontal pathogens.
10.5051/jkape.1999.29.1.265
- Author:
Ha Na JEONG
1
;
Jong Suk OH
;
Young Jun KIM
;
Hyun Ju CHUNG
Author Information
1. Department of Periodontology, College of Dentistry, Chonnam National University, Korea.
- Publication Type:Original Article
- Keywords:
Lactobacillus acidophilus;
periodontal pathogen;
probing pocket depth;
gingival index;
gingival crevicular fluid
- MeSH:
Aggregatibacter actinomycetemcomitans;
Bacteria*;
Bacteroides;
Cell Count;
Gingival Crevicular Fluid;
Humans;
Hydrogen;
Hydrogen Peroxide;
Lactic Acid*;
Lactobacillus acidophilus;
Lactobacillus casei;
Milk;
Periodontal Diseases;
Periodontal Index;
Porphyromonas gingivalis
- From:The Journal of the Korean Academy of Periodontology
1999;29(1):265-276
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to evaluate the effect of hydrogen peroxide-producing Lactobacillus acidophilus V-2Oonthe replication of periodontal pathogens, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. When A. actinomycetemcomitam and P. gingivalis were incubated alone and in the combination with L. acidophilus V-20, the viable cell numbers of A. actinomycetemcomitans and P. gingivalis were compared between those cultures. The effect of S. mutans, E. durans, and L. lactis on the replication of A. actinomycetemcomitans and P. gingivalis was also evaluated. The change of periodontal indexes(probine depth, gingival index, GCF volume) and the viable cell numbers of A. actinomycetemcomitans and black pigmented bdcteroides in subgingival plaque sample were evaluated following gargling of fermented milk made from L. acidophilus V-20 for 1 month on patients with periodontal disease in maintenance phase. In the mixed culture of L. acidophilus V-20 and A. actinomycetemcomitans or P. gingivalis, the replication of A. adinomycetemcomitam or P. gingivalis was completely inhibited. But in the mixed culture of P. gingivalis and hydrogen peroxide-nonproducing Lactobacillus casei, the viable cell numbers of P. gingivalis was not decreased when compared with the numbers in the mixed culture of P. gingivalis and L. acidophilus V-20. In the mixed culture of A. actinomycetemcomitam and S. mutans, E. durans, or L. lactis, the viable cell number of A. adinomycetemcomitans was not almost changed when compared with the numbers in the culture of A. actinomycetemcomitans alone. And in the mixed culture of P. gingivalis and E. durans or L. lactis, the viable cell numbers of P. gingivalis was not almost changed compared with the counts in the culture of P. gingivalis alone. But the replication of P. gingivalis was completely inhibited in the mixed culture of P. gingivalis and S. mutans. When the change of periodontal indexes following gargling of fermented milk was compared with baseline, probing depth and gingival index were not changed, but GCF volume was significantly dcreased(p (0.05). And when the viable cell numbers of microorganisms in subgingival plaque sample were compared with baseline, total viable cell number was almost unchanged and the viable cell numbers of A. actinomycetemcomitans and black pigmented bdcteroides were significantly decreased(p<0.05). These results suggest that L. acidophilus V-20 inhibit the replication of A. actinomycetemcomitans and black pigmented bacteroides by the formation of hydrogen peroxide.
