Differential Expressions of Fas and Fas Ligand in Human Placenta.
10.3346/jkms.2002.17.2.213
- Author:
Cheong Rae ROH
1
;
Jeong Won LEE
;
Byung Hee KANG
;
Soon Ha YANG
;
Byoung Gie KIM
;
Duk Soo BAE
;
Jong Hwa KIM
;
Je Ho LEE
Author Information
1. Department of Obstetrics and Gynecology, Samsung Medical Center, and Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea. crroh@smc.samsung.co.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Fas;
Fas ligand;
Placenta;
Trophoblast
- MeSH:
Antigens, CD95/biosynthesis/*genetics;
Fas Ligand Protein;
*Gene Expression;
Gene Expression Profiling;
Glycosylation;
Humans;
Immunoblotting/methods;
Membrane Glycoproteins/biosynthesis/*genetics;
Placenta/*metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Trophoblasts/cytology/metabolism
- From:Journal of Korean Medical Science
2002;17(2):213-216
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the expressions of Fas and Fas ligand (FasL) in human placenta, we studied the expressions of Fas and FasL in placenta with RT-PCR, immunoblotting and immunostaining. We observed amplified products of Fas and FasL transcripts, the band of Fas (52 kDa) and multiple bands of FasL (42-52 kDa) in pla-centa. Fas and FasL localized mainly on fetal vessels and on syncytiotrophoblasts respectively. The differential distribution of Fas and FasL in human placenta may reflect intrinsic expressions of them by trophoblasts during differentiation. The increased expression of Fas in trophoblasts may promote apoptosis of placenta in pathologic condition such as preeclampsia.
