Optimization of culture media of pathogenic Mycoplasma hyopneumoniae by a response surface methodology.
10.4142/jvs.2010.11.4.327
- Author:
Mi Hyun HWANG
1
;
Dereje DAMTE
;
Min Hee CHO
;
Young Hoan KIM
;
Seung Chun PARK
Author Information
1. College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. parksch@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
fresh yeast extract;
horse serum;
Mycoplasma hyopneumoniae;
optimization;
response surface analysis
- MeSH:
Biotechnology/*methods;
Culture Media/*chemistry;
Mycoplasma hyopneumoniae/*growth & development
- From:Journal of Veterinary Science
2010;11(4):327-332
- CountryRepublic of Korea
- Language:English
-
Abstract:
Composition of culture medium for mass production of Mycoplasma hyopneumoniae was optimized using a response surface methodology (RSM). Initially, the influence of glucose, thallium acetate, fresh yeast extract, horse serum, and porcine serum on the production of mycoplasmal protein was assessed using a 'one factor at a time' technique. Next, factors with a significant effect, including fresh yeast extract, and horse and porcine sera, were selected for further optimization using a central composite design (CCD) of RSM. The experimental results were fitted into a second order polynomial model equation. Estimated optimal condition of the factors for maximum production of mycoplasmal protein (i.e., triple-fold increase from 0.8 mg/L produced by basal mycoplasma media to 2.5 mg/L) was 10.9% fresh yeast extract, 15% horse serum, and 31.5% porcine serum (v/v). For the optimized conditions, a 2.96 mg/L experimental result was observed, similar to the estimated optimal conditions result of the CCD.
