Development of a universal plate-agglutination test for detecting Haemophilus parasuis.
10.4142/jvs.2010.11.4.355
- Author:
Dingqian GUO
1
;
Cheng TANG
;
Quan HAI
;
Guoqing SHAO
;
Hua YUE
Author Information
1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041 China. yhua900@yahoo.com.cn
- Publication Type:Brief Communication
- Keywords:
diagnostic antisera;
Haemophilus parasuis;
plate-agglutination
- MeSH:
Agglutination Tests/*methods;
Animals;
Cross Reactions;
Haemophilus parasuis/isolation & purification/*physiology;
Immune Sera/*metabolism;
Reproducibility of Results;
Sensitivity and Specificity
- From:Journal of Veterinary Science
2010;11(4):355-357
- CountryRepublic of Korea
- Language:English
-
Abstract:
Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.
